Objectives: Lateral canals within the root canal system may contribute to the development of apical periodontitis, which can negatively affect the success of endodontic treatment. The aim of the study was to assess the depth of penetration of AH Plus Bioceramic root canal obturating sealer into lateral canals, using different obturation techniques on 3D-printed teeth. Materials and Methods: The study used 45 3D-printed lateral incisors with a working length of 22 mm, an apical tip diameter of 0.30 mm and a lateral canal diameter of 0.15 mm at a distance of 3 mm from the apex. All canals were prepared with Endoart Touch Gold files to a size of 35/.06. The canals were irrigated with distilled water during preparation. After preparation, the main and lateral canal openings were checked with a #10 K file. Teeth were randomly divided into three groups according to obturation technique: Single cone method (n = 15), cold lateral condensation (n = 15) and warm vertical condensation (n = 15). The teeth were obturated with AH Plus Bioceramic root canal sealer and gutta-percha. After obturation, the teeth were radiographed and scored. The results were statistically analysed using Kruskal-Wallis and Mann-Whitney U tests and a significance level of p < 0.05 was accepted. Results: Different obturation techniques used in the study influenced the penetration of the root canal filling material into the lateral canals (p = 0.002). When the warm vertical compaction technique was used, AH Plus Bioceramic canal sealer penetrated into lateral canals better than the single cone (p = 0.001) and cold lateral condensation (p = 0.010) techniques. Conclusions: The obturation technique used affected the obturation depth of the lateral canals of the AH Plus Bioceramic root canal sealer. The warm vertical condensation technique was found to be more successful than other techniques.