Abstract
The left internal mammary artery is the conduit of choice for coronary artery bypass grafting. In the traditional ("clipped-artery") harvesting technique, this artery is prepared as a pedicle; the distal part is clipped, cut, and covered with a papaverine-soaked cloth until anastomosis Is performed. In modified ("nonclipped-artery') harvesting, the prepared artery is kept in situ and left connected to the systemic circulation until anastomosis. Better outcomes from use of the nonclip technique have been reported. In order to determine comparative endothelial integrity and endothelial nitric oxide synthase activity we performed an immunohistochemical study of arterial graft segments that were procured by each technique. This cross-sectional study involved 40 patients who underwent elective coronary artery bypass grafting. The patients were randomized into 2 groups of 20. One group underwent traditional clipped-artery harvesting; the other group, modified nonclipped-artery harvesting. By immunohistochemical methods, we examined redundant segments taken from bifurcation levels of the arteries. The tunica media was thinner In the clipped arterial segments, a phenomenon that we attribute to high luminal pressure. Endothelial nitric oxide synthase immunostaining was absent in regions of denudation in the luminal endothelia of the clipped arteries; in contrast, pronounced immunostaining occurred in the endothelia of the nonclipped segments. We found that traditional harvesting disrupted the Integrity of the luminal endothelia of the clipped arteries. In addition, the traditional procedure decreased nitric oxide production, as was revealed by immunostaining.
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Kapsamı
Uluslararası
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Type
Hakemli
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Index info
WOS.SCI
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Language
English
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Article Type
None
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Keywords
coronary artery bypass methods coronary disease surgery endothelium, vascular physiology graft occlusion, vascular graft survival immunohistochemistry internal mammary-coronary artery anastomosis adverse effects mammary arteries transplantation myocardial revascularization nitric oxide synthase analysis metabolism type III tunica media enzymology pathology