Background: This study presents the production of a hierarchical porous carbon material (HPC) from rubber wastes obtained from Cilas Rubber Company operating in Zonguldak-Devrek/T & uuml;rkiye, and use of the HPC in operation of a phenol biosensor. Methods: Molten Salt method, which is an environmental-friendly progress, was utilized for the carbonization process. Chitosan polymer was modified with HPC to form a film on the glassy carbon electrode surface and laccase was chemically immobilized onto this film. The electrode was then tested for phenol detection at +0.44 V by using differential pulse voltammetry. Significant findings: Various pore sizes were formed due to salt aggregation with Molten Salt method in the carbon matrice. The biosensor presented a linear range from 30 mu M (2.82 ppm) to 300 mu M (28.2 ppm), a LOD of 10.45 mu M, a measurement accuracy of 99.38 % +/- 3.62 with the RSD of 4.46 %. %. The amounts of electroactive laccase for the HPC-modified and unmodified electrodes were calculated to be 116 mu g and 32 mu g, respectively. The biosensor accurately measured phenol in tap and stream water, achieving accuracies of 97.7 % and 92.3 %, respectively. HPC amplified the biosensor signal 1.83 times higher in comparison to unmodified electrode. Rubber wastes were evaluated as raw materials in a production process. A porous carbon material produced from rubber waste was used as a biosensor electrode for the first time.