Aim This study was planned with a multi-centre design to investigate the mediator role of person-organization fit in the relationship between organizational agility and individual work performance in nurse managers. Method This multi-centre, descriptive cross-sectional study was carried out with 169 nurse managers working at four public hospitals and two university hospitals operating in two provincial centres. Data were collected face-to-face between January 2025 and March 2025 using a 'Personal Information Form,' the 'Person-Organization Fit Scale,' the 'Individual Work Performance Questionnaire,' and the 'Organizational Agility Scale.' Data were analysed using the SPSS Statistics 30.0 programme and the PROCESS Macro v4.2 extension with frequency, percentage, mean and standard deviation values, t-tests, Kruskal-Wallis tests and Pearson's correlation analyses. Results Organizational agility had a significant and positive effect on person-organization fit (B = 0.811, SE = 0.068, p < 0.05). Person-organization fit significantly predicted individual work performance (B = 0.190, SE = 0.062, p < 0.05). The indirect effect of organizational agility on individual work performance was also significant (B = 0.154, SE = 0.049, 95% CI: 0.053-0.246). Therefore, person-organization fit mediated the relationship between organizational agility and individual work performance. Conclusion This study demonstrated the mediator role of person-organization fit in the relationship between organizational agility and individual work performance in nurse managers. The results of the study showed that an increase in organizational agility indirectly and positively affected individual work performance via the mediation of person-organization fit.