This article presents for the first time the scientific measurement and knowledge network analysis of the development and evolution of enzymatic fuel cell (EFC) technology in the past 41 years, based on information from 980 relevant articles in the Web of Science (WoS) Core Collection. WoS data have been edited for "Biblioshiny Software" using "R Programming" and then these edited data have been transferred to "Biblioshiny Software". The results showed that the EFC research field exploded in 2017. The USA was the country with the strongest collaboration, and the author of the most cited article globally is Zebda A. The core findings from the analysis results showed that the research area was focused on the design of biobatteries, supercapacitors, micro- EFC designs, wearable and self-powered biosensors in last 4 years. Also, EFC with dual-mode sensing and EFCs developed for use as tumor biomarkers are new research areas. This article will help readers gain a quick perspective on the research structure and future directions by mapping the research knowledge on EFC through bibliometric analysis.