Vitamin D-3 is a fat-soluble vitamin synthesized in the skin from 7-dehydrocholesterol and activated some hydroxylase enzymes in liver and kidney. Cytochrome P450 enzymes are a group of essential enzymes in the body responsible for metabolizing a wide range of substances, including drugs, toxins, and hormones. They play a vital role in detoxification, drug metabolism, and the synthesis of critical compounds. Vitamin D is critically essential for not only tumorigenesis but also cancer treatment and drug rsistance. Expression imbalance in the level of CYP24A1 has been observed in several cancer types such as adenocarcinoma, breast carcinoma, and colon cancer. CYP24A1 trigggers the deficiency of vitamin D3 through catalizing the production of lactone or calcitroic acid.