Glyphosate (Gly), a widely used herbicide, is a significant environmental pollutant that causes water pollution and human toxicological effects resulting from intensive agricultural practices. This study offers a rapid and efficient electroanalytical approach for detecting Gly in various water sources, utilizing SrxZn1-xO/g-C3N4 composite materials. The SrxZn1-xO solid solution, which exhibits a hexagonal wurtzite structure, was synthesized through a sol-gel method followed by a hydrothermal treatment. The synthesized SrxZn1-xO and g-C3N4 structures were characterized using XRD, FTIR, XPS and SEM techniques. Electrochemical evaluation of the SrxZn1-xO/g-C3N4 electrodes revealed enhanced charge transfer properties and a high density of active sites, particularly for the Sr0.025Zn0.975O/g-C3N4 configuration. This electrode demonstrated effective detection of Gly via electrochemical reduction, using differential pulse voltammetry (DPV) at a potential of-0.719 V vs. Ag/AgCl in phosphate buffer (pH 7), without requiring any pre-treatment steps. The DPV response exhibited linearity across two concentration ranges: The concentration ranges from 0 to 10 nM and from 10 to 100 nM. The method demonstrated an impressive limit of detection (LOD) of 0.14 nM and a notable limit of quantification (LOQ) of 0.45 nM, signifying exceptional sensitivity. The application of the Sr0.025Zn0.975O/g-C3N4 sensor to real-world water samples yielded Gly concentrations of 4.1 +/- 0.29 nM in Sapanca Lake water, 7.3 +/- 0.45 nM in natural spring water, and 2.9 +/- 0.33 M in tap water-all collected from T & uuml;rkiye. These results present innovative ap-proaches for the accurate, rapid, practical and low detection limit analysis of Gly contamination of the developed sensor and confirm its potential for in situ environmental monitoring.