Background: This study was conducted to determine the effect of yoga practice on fear of childbirth and childbirth self-efficacy. Materials and methods: This study was performed in a quasi-experimental model with pretest and post-test control groups. The participants of the study were women who applied to a pregnancy education class at a hospital. Ninety women participated in the study. The data used in the study were collected using the Individual Information Form, the Wijma Delivery Expectation/Experience Scale (Version A), and the Childbirth Self-Efficacy Scale. Results: After participating in yoga practice, the pregnant women in the experimental group exhibited decreased fear of childbirth and increased childbirth self-efficacy (p < .05). On the other hand, the pregnant women in the control group displayed increased fear of childbirth and decreased childbirth self-efficacy (p < .05). Conclusion: Based on the results of this study, yoga practice reduces fear of childbirth and significantly increases childbirth self-efficacy.