In this paper, by using the Golden Calculus, we introduce the generalized Apostol-type Frobenius-Euler-Fibonacci polynomials and numbers; additionally, we obtain various fundamental identities and properties associated with these polynomials and numbers, such as summation theorems, difference equations, derivative properties, recurrence relations, and more. Subsequently, we present summation formulas, Stirling-Fibonacci numbers of the second kind, and relationships for these polynomials and numbers. Finally, we define the new family of the generalized Apostol-type Frobenius-Euler-Fibonacci matrix and obtain some factorizations of this newly established matrix. Using Mathematica, the computational formulae and graphical representation for the mentioned polynomials are obtained.