Aim: Burn injuries are one of the most devastating injuries. Saving the zone of stasis de-creases burn size, morbidity, and mortality. Tarantula cubensis (TC) increases epitheliza-tion of wounds, and increases wound contraction. In this study, we investigated the effects of TC on the stasis zone.Materials and Methods: 36 Wistar albino female rats were divided into 3 groups. Using brass comb burn model, at days 0 and 3, physiological serum solution in group 2, TC injection in group 3and no injection in group 1 was applied. Thermal and normal images were taken on day 10 and rats were sacrificed for histopathological examination.Results: We found a statistically significant difference between the 1st and 3rd group, 2nd and 3rd group regarding viable wound areas (p < 0.05), temperature difference (body-stasis zone) and mean body temperature (p < 0.05). We found no statistically significant dif-ference between groups regarding neovascularization, inflammatory density, and vital cutaneous appendages. We found a statistically significant difference in epithelial layer thickness between the1st and 3rd groups, 2nd and 3rd groups(p < 0.05).Conclusion: TC increases stasis zone viability macroscopically, increases epithelial layer thickness histopathologically, thus it promotes wound healing in burn wounds. This in-crease in stasis zone viability was also established with thermal imaging (c) 2022 Elsevier Ltd and ISBI. All rights reserved.