Objective: An important criterion that radiopaque poly-3-hydroxy butyrate (PHB) derivatives synthesized and characterized by our study group do not interact with DNA and bovine albumin to be developed as an intravenous contrast agent for radiological imaging.,Methods: Interactions of radiopaque PHB derivatives between bovine serum albumin (BSA) and deoxyribonucleic acid (pUC19 DNA and polymerase chain reaction product) were investigated using absorption spectroscopy and agarose gel electrophoresis techniques.,Results: Absorption spectroscopy results showed that the radiopaque PHB derivatives did not show any interaction with BSA. Double-stranded annular DNA showed that the interaction between the double-stranded forms of linear DNA did not cause any damage.,Conclusion: It was determined that it did not interact with both BSA and various DNA forms.