Multi response optimisation conditions were investigated in grape seeds' phenolic compounds extraction by using ultrasound-assisted extraction (UAE) methodology. The effect of independent process variables such as EtOH concentration (0-100%), extraction time (0-40min), solvent:solid ratio (4.5-38.5mL/g) and extraction temperature (20-60 degrees C) on total phenolic content (TPC) and total antioxidant activity (TAA) of the extracts were studied. The optimum conditions of UAE were determined as follows: EtOH concentration, 61.76%; extraction time 20min., solvent:solid ratio, 30mL/g; extraction temperature 50 degrees C. The estimation results of the model and the experimental results for TPC and TAA showed a great similarity.