Newly synthesized poly(styrene-g-oleic amide) was coated onto a rhodium nanoparticle modified glassy carbon (GC) surface for the fabrication of horseradish peroxidase based biosensor used for hydrogen peroxide detection. The rhodium modifed electrode presented ten times higher signal than unmodified electrode even at low elecrtroactive enzyme quantity by enhancing the electron transfer rate at the applied potential of -0.65V. The biosensor designed by under the optimized rhodium electrodeposition time exhibited a fast response less than 5 s, an excellent operational stability with a relative standard deviation of 0.6% (n=6), an accuracy of 96% and a large linear range between 50 mu M and 120mM for hydrogen peroxide. Detection limit and the sensitivity parameters were calculated to be 44 mu M and 57 mu A mM(-1)cm(-2), respectively by preserving its entire initial response up to the 15days, while only 20% of its initial response was lost at the end of one month.