ObjectivesThis study is evaluating fetuin-A, serum amyloid A (SAA) and tumor necrosis factor alpha (TNF-) levels in gingival crevicular fluid (GCF) and serum samples in periodontal health and disease.,Material and MethodsSixty patients were divided into three groups: Group 1 periodontal health (n=20), Group 2 gingivitis (n=20) and Group 3 chronic periodontitis (CP) (n=20). GCF and serum samples were evaluated using enzyme-linked immunosorbent assay kit.,ResultsSAA and TNF- levels in GCF and serum were significantly higher in patients with gingivitis and CP compared with controls (P<0.016). Contrarily, fetuin-A levels in GCF and serum were significantly higher in controls than in patients with gingivitis and CP (P<0.016). In CP group, a significant correlation was observed between GCF-SAA amount and the number of sites with 4PPD5mm (P<0.05). There was a significant correlation between GCF-fetuin-A levels and the number of sites with PPD6mm (P<0.05). GCF-TNF- was found to have a significant relationship with the number of sites with 4PPD5mm and PPD6mm (P<0.05).,ConclusionsIn conclusion, serum and total levels of SAA significantly increased, whereas fetuin-A levels significantly decreased, with increasing severity of PD.