Objective: This study aims to investigate the prevalence of rotavirus (RV) and adenoviruses (EAV) during different seasons in children aged between 0 and 14 years who were diagnosed with acute gastroenteritis.,Materials and Methods: In 2014, the prevalence of rotavirus and adenovirus antigens was investigated using immunochromatographic methods, i.e., rotavirus and adenovirus antigen kits (Ameritek one step rapid test adenovirus/rotavirus complex 2 - panel card test), on 3258 patients who provided their stool samples and were diagnosed with acute gastroenteritis on admission to our hospital. Data were retroactively obtained from the laboratory database.,Results: The viral antigen was detected in 638 (19.6 %) of 3258 patients. Rotavirus was detected in 590 (18.1 %) samples and adenovirus was detected in 48 (1.5 %). Furthermore, this study demonstrated that rotavirus antigen positivity was higher in the spring, while enteric adenovirus positivity was higher in the summer.,Conclusion: It is important to clinically identify the prevalence of rotavirus and enteric adenovirus antigens in stool samples. Moreover, it is considered a necessity to routinely investigate rotavirus and enteric adenovirus antigens because of climate conditions in our region which are susceptible for gastroenteritis cases.