The medical drama series, Masters of Sex, started to be aired in 2013 and completed its first season with twelve episodes. It is an intriguing production telling the story of William H. Masters and Virginia E. Johnson in details as well as displaying the "Masters and Johnson Sex Research". Although the series aims at showing the details of this research conducted under difficult conditions, it gives insights into women's life experiences in that period. This article focuses on the conditions of women in the USA in the 1950s through an analysis of the TV series, Masters of Sex. The analysis is based on four female characters: Libby Masters who dedicates herself to have a baby for a better and happier life; Margaret Scully who is late for sexual experience because of her gay husband; Lillian DePaul who acts masculine in order not to reveal her womanhood in her job and researches; and a single mother, Virginia Johnson, leaving the other characters behind with her free spirit. This article addresses the media representation of the characters living in the USA in the 1950s and examines them through the issues of motherhood, sexuality and body by drawing on a feminist perspective.