Senecio genus includes taxa that can be used for agricultural, pharmacological, physiological and biochemical studies, on account of their different pyrrolizidine alkaloids (PAs). Senecio hypochionaeus var. argaeus is an endemic species in Turkey. Successful callus production was achieved in all the N(6)-benzyladenine (BA) and naphthaleneacetic acid (NAA) experiments in the root explants. The highest level of shoot generation in the apical meristem explants was observed in Murashige and Skoog medium that contained 1 mg/l BA and 1 mg/l NAA. The best root formation was obtained from 8 mg/l indolebutyric acid. Young rooted shoots were transplanted to pots with sterile garden soil, and then grown in an acclimatization room.