The equilibrium and the kinetics of BSA adsorption onto specific surface area changed TiO2 by heat treatment were studied. The TiO2 was treated at 100 and 200 degrees C for 24 h. The specific surface areas of TiO2 characterized by BET method were measured as 48.9 m(2)/g and 53.0 m(2)/g for 100 and 200 degrees C, respectively. The adsorption rate and the equilibrium experiments were carried out at pH 4 for 40 degrees C. The rate kinetics for the adsorption of BSA was best fitted with the pseudo-first-order kinetic model. The equilibrium process was described by the Langmuir and Freundlich isotherm models. The adsorption capacities (Q(0)) calculated from the Langmuir isotherm model were 40.6 and 44.4 mg/g for heat-treated TiO2 at 100 and 200 degrees C, respectively. The adsorption of BSA increased with increasing surface area of TiO2. The zeta potential values of the 100- and 200 degrees C-treated TiO2 were found as -2.57 mV and 0.39 mV, respectively, showing that the interaction between TiO2 with BSA increased with increasing temperature of heat treatment for TiO2. (C) 2009 Elsevier B.V. All rights reserved.