Total lipid amount and fatty acid composition were determined and compared for cultivated European sea bass (Dicentrarchus labrax) from Turkey, which were fed with pellet and extruded commercial feed two or three times a day. Fatty acid analyses were carried out by gas chromatography-mass spectrometry. Among total fatty acids, palmitic (16:0), palmitoleic (16:1n-7), oleic (18:1n-9), linoleic (18:2n-6), eicosapentaenoic acid = EPA (20:5n-3) and docosahexaenoic acid = DHA (22:6n-3) were found to be the predominant fatty acids. The fish which received extruded feed (three times a day) had a slightly higher n-3/n-6 ratio of 1.41 compared to the other feeding types. It was found that the extruded feed given two times a day as fish diet resulted in the highest amounts of EPA, DHA, and total n-3 fatty acids.